Typical ELISA run at Argutus Medical
Tony Loughman PhD prepares urine samples
Tony loads the plates
Accurate dispensing is critical
Following initial incubation plates are washed
It is very important to have your washer optimized for the plates being washed
After washing the plates should be tapped dry if any residual liquid remains
A multichannel pipette is used for aspirating conjugate
All wells receive conjugate
Following incubation, wash, substrate addition and a further incubation stop solution can be added to terminate the assay
Clear color change is observed in the calibrators