Assay Principle

The Argutus Medical Serum Collagen IV EIA is designed for the assay of serum collagen IV. It is a solid phase one-step sandwich EIA. Collagen IV in the sample is bound simultaneously by a solid-phase monoclonal antibody and a monoclonal antibody-enzyme conjugate, each directed at different antigenic sites. This results in the collagen IV molecule being sandwiched between the solid phase and enzyme-labelled antibodies. After removing unbound enzyme-labelled antibody and sample, the plate is incubated with enzyme substrate. The resultant colour development is directly proportional to the amount of collagen IV in the sample.

Performance Characteristics

MEASURING RANGE
The calibration curve range covers the range 15.6-1000 μg/L. This range may be extended by increasing sample dilution.

SPECIFICITY
The Argutus Medical Serum Collagen EIA IV is highly specific for the detection of collagen IV. Cross reactivity is less than 2% with Collagen II and less than 0.5% with other forms of collagen.

SENSITIVITY
When reading from the standard curve the A450nm value of the 1000 μg/L standard should be >0.6 OD.

For more information on this product and it's usage see the links to product documentation on this page. Alternatively, feel free to contact us