Summary

• The Argutus Medical OxyDNA Test can be used to study oxidative DNA damage resulting from many environmental toxins.

Selected Articles

1) The role of oxidative stress in the ochratoxin A-mediated toxicity in proximal tubular cells.
Schaaf, G et al. (2002). Biochim. Biophys. Acta. 1588(2) 149-58.

• Exposure to the fungal toxin Ochratoxin caused oxidative DNA damage.
• Addition of the anti-oxidant N-acetyl cysteine reduced DNA oxidation.
• Ochratoxin is suspected in being involved in the pathology of endemic Balkan nephropathy.

2) Oxidative DNA damage in peripheral leukocytes and its association with expression and polymorphisms of hOGG1. A study of adolescents in a high risk region for hepatocellular carcinoma in China.
Shen, H.-M., Liu, Z.-M., Yan, L.-N., Peng, M.-H., Li, L.-Q. Liang, R.-X., Wei, Z.-L. Halliwell, B. and Ong, C.N. (2003).
World J. Gastroenterol. 9(10) 2186-2193.

• OxyDNA correlated highly with the DNA repair enzyme hOGG1.
• Reproducibility of the Argutus Medical OxyDNA Test was better than 90% and variability under 10%.

3)  Protective effect of metallothioneins against oxidative stress evaluated on wild type and MT-null cell lines by means of flow cytometry
Colangelo, D., Mahboobi, H., Viarengo, A. and Osella, D. (2004). Biometals. 17(4) 365-70.

• Metallothionins protect cells against cadmium induced oxidative DNA damage.

4) Cell type-specific effects of asbestos on intracellular ROS levels, DNA oxidation and G1 cell cycle checkpoint
Kopnin, P.B., Kravchenko, I.V., Furalyov, V.A., Pylev, L.N. and Kopnin, B. P. Oncogene 23(54) 8834–8840.

• The Argutus Medical OxyDNA Test can distinguish between the relative sensitivities of fibroblasts and mesothelial cells to chrysotile.
• 8-oxodeoxyguanine staining increases in dose response fashion to exposure to chrysotile.