Summary
- Elevated perfusate αGST can predict poor graft function.
- Perfusate αGST levels correlate with histological injury.
- Perfusate αGST can be used to compare graft storage and transport systems
1) Improved rat liver preservation by hypothermic continuous machine perfusion using Polysol, a new, enriched preservation solution
Bessems, M., Doorschodt, B.M., van Vliet, A.K. and van Gulik, T.M. (2005). Liver Transpl. 11(5) 539-46.
- Perfusate αGST levels were lower following perfusion with Polysol than with UW solution.
2) Value of α glutathione S-transferase for in-vitro evaluation of preservation injury in normal and steatotic livers.
Minor, T. et al. (2000). Transplant International 13 (Suppl. 1) 551-554.
- Isolated livers from 5 donor rats with fatty livers and 5 controls were perfused, then transplanted.
- αGST could identify those grafts at risk of failure.
- αGST was the most sensitive parameter for distinguishing between healthy and suboptimal grafts.
- αGST and GLDH was a particularly useful combination.
3) Value of alpha-glutathione sulfotransferase in the assessment of. hepatic injury after experimental long-term preservation.
Cascales, P., Fernández-Cornejo, V.J., Sánchez-del Campo, F., de Torre, M., Sánchez Eixeres, M.R., Soriano, N. and González F. (1999). Transplant Proc. 31(6) 2435-6.
- 23 rat livers studied.
- Perfusate αGST levels correlated more closely with histologically evaluated liver injury than ALT, AST and LDH.
4) Prediction of severe reperfusion injury by aGST concentration in graft perfusates.
Viebahn, R. et al. (1996). Abstract presented at XVI congress of the Transplantation Society, Barcelona, August 25-30, 1996.
- 50 liver transplants in 43 patients.
- All 10 graft failures were associated with elevated αGST in the perfusate.