Human Basal Membrane - HypII108C10

This is a monoclonal mouse IgG antibody directed against a component in the basal membranes. In the picture the reaction with basal membranes of tubules of human kidney is shown



In the image below the basal membranes are stained with HypII108C10 anti human basal membrane antibody in indirect IF with a AlexaFluor 595 labeled anti mouse IgG1 antibody
NiCaII13 indirect IF with AlexaFluor 488 goat anti mouse anti IgG3 (gamma 3-specific). It is clearly shown that on the normal kidney slice the cuboid collecting duct cells are "based" directly on the basal membranes.




The next staining pattern is on a slice of human renal carcinoma. In this part of the renal carcinoma collecting duct-like structures are seen ("Collecting Duct Carcinoma"). The staining with the two antibodies is the same as before. On the carcinoma it is clearly seen that the "colleciting duct-like carcinoma cells" are not based directly on the basal membranes




In this slice we found infiltrating tumor tissue within the normal part of the kidney.
Staining was performed for basal membranes with HypII108C10 and with PMII29 for distal tubules. The little green spots could be membranes of capillaries in the tumor




In this picture, the staining of distal tubules with PMII29 was contrasted with the staining of basal membranes.




In this last picture proximal tubules, stained with PMII39 are contrasted with the basal membrane staining of HypII108C10. You can see the large proximal tubules close to the glomerulus (the glomerulus is also stained with HypII108 because it contains basal membranes in the capillary filtration apparatus). In addition the Bowman's capsule (around the glomerulus) is stained.




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