In this image we have double-stained a slice of human kidney with the monoclonal anti human distal tubules PMII29D2 antibody (red) and anti human Tamm Horsfall Protein (THP, green).
The picture clearly shows:
- That most of the tubules contain both antigens.
- That in these cases, the PMII29D2 antigen is mainly localized to the basal parts of the tubule cells.
- In contrast THP is localized over the entire cellular area.
That is why the staining is yellow/orange (green & red = orange).
- In addition, some tubules contain solely the PMII29D2 recognized antigen.
Conclusion: PMII29D2 does not react with THP and is detecting a different antigen.
The below is a close up of the above
The below picture belongs to the same series of micrographs in which we have tried to characterizes the specificity of the anti human distal tubule antibody PMII29D2 in relation to THP (stained with a polyclonal commercial anti THP antibody). Here it is done with a larger piece of human kidney.
It shows that the antigens recognized by the two antibodies:
- PMII29D2, red and anti THP green are localized in different tubules.
This picture shows that in this part of the human kidney the green staining by anti THP is not overlapping with the red staining of the PMII29D2-recognized antigen.
In this slice we found infiltrating tumor tissue within the normal part of the kidney.
Staining was performed for basal membranes with HypII108C10 and with PMII29 for distal tubules. The little green spots could be membranes of capillaries in the tumor
In this picture, the staining of distal tubules with PMII29 was contrasted with the staining of basal membranes.
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