In-Vitro Nephrotoxicity

Summary
  • Alpha GST (αGST) and Pi GST (πGST) are localised to distinct parts of the nephron.
  • By the assay of a panel of biomarkers, injury to different parts of the nephron can be studied independently and simulataneously.
  • In-vitro studies can indicate preclinical and clinical toxicity.

 

Selected Articles

 

1) Use of human organ slices to evaluate the biotransformation and drug-induced side-effects of pharmaceuticals.
Vickers, A.E.M. (1994). Cell Biology and Toxicology 10, 407-414.

  • The release of αGST from tissue slices can distinguish the relative toxicities of drug candidates.

 

2) Changes in human liver and kidney slice function related to potential side-effects in the presence of biotransformation of 4 cyclosporin derivatives, CSA, IMM. OG and PSC.
Vickers, A.E.M. et al. (1998) In-vitro and Molecular Toxicology 11(2), 119-131.
 

  • The simultaneous assay of αGST and πGST, enables the proximal and distal tubules to be studied independently.
     

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