Summary
- In-vitro toxicology studies can predict toxicity in rodents and humans.
- Compounds can be ranked by probable toxicity.
- GSTs can be used to study toxicity in both cell cultures and tissue slices.
- αGST is a translatable biomarker from in-vitro to rodents to man.
- By measuring both αGST and πGST, the hepatocytes and the biliary tree can be studied independently.
Selected Articles
Cell Culture
- αGST is an accurate biomarker of increased cell permeability or cytolysis.
- Background release of αGST is very low, making it easier to detect slight increases.
- By measuring αGST, as part of a panel of different biomarkers, a close estimate of in-vivo toxicity (rat and man) can be obtained from in-vitro studies.
- αGST is a translatable biomarker to rodents and man.
1) Oxidative stress in drug discovery: A panel of in vitro toxicity markers
Mitsikosta, F., Fornasier, M. and Westerberg, G. Poster presented at 45th Eurotox Annual Meeting, Rhodes, Greece, October 5-8 2008.
- αGST is an excellent biomarker for studying oxidative stress in human cell lines
2) A new approach to in vitro toxicity screening based on multi-endpoint analysis provides information on mechanism and predicts in vivo toxicity.
McKim, Jr., J.M. et al. (2001). Poster presented at the 40th annual meeting of the Society of Toxicology, San Francisco, March 25-29, 2001.
3) Characterization of the rat α-glutathione S-transferase (GST) ELISA (Biotrin) using rat hepatoma (H4IIE) cells.
Wilga, P.C. et al. (2001). Characterization of the rat α-glutathione S-transferase (GST) ELISA (Biotrin) using rat hepatoma (H4IIE) cells. Poster presented at the 40th annual meeting of the Society of Toxicology, San Francisco, March 25-29, 2001.
- By measuring αGST, as part of a panel of different biomarkers, a close estimate of in-vivo toxicity (rat and man) can be obtained from in-vitro studies.
- The Argutus Medical Rat Alpha GST EIA is very reproducible.
- Provides methodological details on the assay of αGST in cell cultures.
Liver Slices
1) Use of human organ slices to evaluate the biotransformation and drug-induced side-effects of pharmaceuticals.
Vickers, A.E.M. (1994). Cell Biology and Toxicology 10 407-414.
- Illustrates that release of αGST from liver slices can distinguish the relative toxicities of drug candidates.
2) Changes in human liver and kidney slice function related to potential side-effects in the presence of biotransformation of 4 cyclosporin derivatives, CSA, IMM. OG and PSC.
Vickers, A.E.M. et al. (1998). In-vitro and Molecular Toxicology 11(2) 119-131.
- This article shows how by the simultaneous assay of αGST and πGST, the hepatocytes and biliary trees can be studied independently