Currently, GSTs are the most sensitive and region-specific biomarkers for the detection of tubular injury. GSTs are found in high concentrations in the cells of the renal tubules. They are readily released into the urine during injury and, thus, are excellent biomarkers for tubular injury resulting from nephrotoxicity, ischaemic injury, transplant rejection, proteinuria and diabetic renal tubular injury. Different GST subclasses are localized in specific parts of the renal tubule: α-GST in the proximal tubule and Pi-GST in the distal tubule. The specific presence of these subclasses in urine is an early indicator of the site and extent of renal injury. By monitoring the release of different GST subclasses following exposure to potential nephrotoxins the site and extent of tubular injury can be identified (Nephron 2000 85:120–6, Arch Toxicol 1998; 72:104–9, Antimicrob Agents Chemother 2005; 49: 3784–8, Life Sci 2005 18; 76:2125–35).
Alpha GST and Renal Injury
Alpha Glutathione S-transferase (GST) comprises up to 5% of the soluble protein in the proximal renal tubules from which it is rapidly released in response to injury. Urinary alpha GST is a sensitive and specific biomarker for proximal tubular injury, the commonest form of nephrotoxicity found during drug development. Urinary Alpha GST has proven to be an extremely useful biomarker with major applications in clinical research and human clinical trials, from phase I safety up to, and including, large global multicenter phase III trials. Matching assays for rat and in-vitro samples enable easy comparison between experimental and clinical studies
Argutus Medical NEPHKIT Urinary Alpha GST EIA
The Argutus Medical Urinary Alpha GST EIA is a quantitative enzyme immunoassay for the assay of alpha GST in human urine. For the assay of alpha GST in other tissue fluids and species, contact Argutus Medical for advice.
The test procedure is based on the sequential addition of sample, anti alpha GST enzyme conjugate and substrate to microassay wells coated with anti alpha GST. The resultant colour intensity is proportional to the amount of alpha GST present in the sample. The assay range is 0-40ug/L, equivalent to 0-100ug/L in stabilized samples diluted 1:2, and total assay time is 2 hours.
Kit Inserts & MSDS
Further Alpha GST Information
Argutus Medical have a long history of assay development with alpha GST and have produced kits in the past for canine and porcine pre-clinical models. The canine and porcine kits are not currently available from Argutus Medical but please don't hesitate to contact us should you have any questions regarding them please contact us on info@argutusmed.com. For information on the Argutus rat alpha GST kits please click here