Neuronal Injury

Summary

• Oxidative DNA damage can be detected in both cell cultures and tissue sections.
• Degerative neural diseases are assocated with increased staining for oxidised DNA.

 

Selected Articles

 

1) Folic acid deficiency and homocysteine impair DNA repair in hippocampal neurons and sensitise them to amyloid toxicity in experimental models of Alzheimer’s disease
Kruman I.N. et al. (2002). The Journal of Neuroscience 22(5) 1752-1762.

• Reduced vitamin content results in increased neuronal sensitivity to oxidative DNA damage

 

2) DNA is a target of the photodynamic effects elicited in A2E laden RPE by blue light illumination
Sparrow J.R. et al. (2003). Investigative Ophthalmology and Visual Science (14)5 2245-2251.

• Exposure to cultured retinal cells to light can result in oxidative DNA damage. The inhibition of theis by azide indicates that singlet oxygen production is involved.

 

3) Lithium inhibits Aβ-induced stress in endoplasmic reticulum of rabbit hippocampus but does not prevent oxidative damage and tau phosphorylation
Ghribi, O., Herman, M.M. and Savory, J. (2003). Journal of Neuroscience Research 71(6) 853 – 862.

• Beta amyloid induces oxidative stress including oxidative DNA damage

 

4) Using cryopreserved neuronal cells for assessment of chemically induced oxidative cell stress in primary cell culture
Krantis, A. et al. 2003. Poster presented at the 42nd meeting of the Society of Toxicology, March 21-25 2003, Nashville, Tennessee, USA.

• Neuronal cultures could be used to study the effect of potential oxidizing agents in-vitro.