The Argutus Medical OxyDNA Test is an in vitro fluorescent technique for detecting oxidative DNA damage. Oxidative DNA damage is involved in many pathological processes. The probe in the Argutus Medical OxyDNA Test, binds to oxidised guanine, the most mutagenic form of oxidative damage. It has been widely used to study oxidative DNA damage in toxicology, environmental medicine, diabetes, neural disease and many other conditions where oxidative DNA damage may occur. | |
Applications
- Toxicology
- Environmental toxicology
- Radiation
- Xenobiotics
- Diabetes
- Cancer
- Inflammation
- Chronic Brain Disease
The OxyDNA Test
The test procedure is based on addition of a fluorescent labelled 8-oxoguanine binding protein to oxidised DNA in fixed cells.
The presence of oxidised DNA is indicated by a yellow fluorescence. The reagents can be used with cell cultures or cell suspensions using the FACS technique.
Kit Insert & MSDS
Further Information on the Argutus Medical OxyDNA Test
Oxidative DNA damage can result from the toxic effects of endogenous metabolites, natural toxins or xenobiotics (e.g. new drugs). The importance of monitoring oxidative DNA damage is clear, but previous methods for detecting it have been laborious and have slowed the development of this field. Argutus has developed a sensitive, specific and convenient method for detecting oxidative DNA damage - the OxyDNA Test - that will expand the possibilities of research. The following review will present the role of oxidative DNA damage in biology and disease processes and its detection using the OxyDNA Test.
DNA damage can be directly lethal to the cell, but more insidious and, maybe more serious, is the incomplete or incorrect repair of DNA lesions leading to mutagenesis or carcinogenesis. The association of 8-oxoguanine formation with mutation makes it a particularly important biomarker of oxidative DNA damage.
It is important to avoid drugs that have the potential to induce DNA damage. For example, Thalidomide has been shown to cause oxidative DNA damage in rabbit foetuses
The OxyDNA Test is a fluorescent-labelled protein binding method for the direct detection of oxidative DNA damage in-situ. It is based on the use of a naturally occurring binding protein with a high specificity and avidity for 8-oxoguanine.
- By binding to the oxidised DNA via the 8-oxoguanine adduct, the OxyDNA Test provides a very sensitive and specific test for oxidative DNA damage. It also provides information on the mechanism of DNA damage.
- The cells are fixed and permeabilised before being incubated with the OxyDNA Test probe. After washing, the cells can be studied using the FACS techniques. Increasing levels of DNA damage is associated with increasing fluorescence. This is shown in figure 2 for lymphocytes exposed to methylene blue plus
light.