Intended Use
Pi glutathione S-transferase (Pi-GST) is found in the distal tubules of the the human nephron. Urinary pi-GST is a sensitive and specific biomarker for renal distal tubular injury. Pi-GST is the only commercially available urianry biomarker for distal tubule injury, an under studied organ where injury can have severe consequences.
Urinary Pi-GST measurements are widely used in human safety testing in pharmaceutical R&D. Furhermore, Argutus Medical is currently conducting comprehensive clinical trials following some very encouraging research indicating a significant clinical value in acute kidney injury (AKI) detection. The complementary rat urinary GSTYb1 EIA enables easy comparison between experimental and clinical studies
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Argutus Medical Pi GST EIA
The Argutus Medical Pi-GST EIA is a quantitative enzyme inmmunoassay for the assay of pi-GST in human urine. For the assay of pi-GST in other biological material please contact Argutus for assistance and advice at info@argutusmed.com
The test procedure is based on the sequential addition of sample, anti pi-GST enzyme conjugate, and substrate to microassay wells coated with anti pi-GST. The resultant colour intensity is proportional to the amount of pi-GST present in the sample. The assay range is 1.25 - 40 ug/L, equivalent to 3.12 - 100 ug/L in stabilised urine samples diluted 1/2. The total assay time is 2.5 hours.
Please
click here to see some recent Argutus Medical publications on Pi-GST
Kit Inserts & MSDS
Further Information
Pi-GST is specific to the distal tubules of the human nephron. Another form of GST, GSTYb1 (muGST), has a similar distribution in rats.Please click here for more information