Intended Use
Alpha glutathione S-transferase accounts for approximately 4% of the soluble protein in hepatocytes and is rapidly released in response to acute hepatocyte injury. Similarly it is rapidily cleared from circulation upon hepatocyte recovery due to its short half life of around 90 minutes. Therefore, serum alpha GST is one of the most sensitive and specific biomarkers for hepatocyte injury currently commercially available
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Assay Principle
The Argutus Rat Alpha GST EIA is a quantitative enzyme immunoassay for the assay of alpha GST in rat urine, serum or tissue culture fluids. For the assay of alpha GST in other materials and species, contact Argutus Medical for advice.
The test procedure is based on the sequential addition of sample, anti alpha GST-enzyme conjugate, and substrate to microassay wells coated with anti alpha GST. The resultant colour intensity is proportional to the amount of alpha GST present in the sample. Assay range is 0-100 microg/L, equivalent to 0-625 microg/L in stabilised urine samples diluted 1/5, and 0-5000 microg/L in serum samples diluted 1/50. Total assay time is 2 ¼ hours.
Kit Insert & MSDS
