Collagen IV and Liver Fibrosis
Collagen IV forms part of the
basal lamina and is released during their turnover and deterioration. Serum Collagen IV is an especially sensitive biomarker for collagen deposition in active liver fibrosis. The Argutus Collagen IV EIA is a simple, but sensitive, method for studying fibrotic changes in the liver. Serum collagen IV levels correlate with the extent of liver fibrosis.
Applications
Hepatotoxicity
- Viral Hepatitis
- Alcoholism
- Liver Cirrhosis
- Chronic Liver Disease
- Hepatocellular Carcinoma
Argutus Medical Serum Collagen IV EIA
In the test procedure, samples are mixed with labelled anti-collagen IV enzyme conjugate. and then transferred to microassay wells coated with anti-collagen IV where the collagen IV enzyme conjugates complexes are captured. After incubating, the wells are washed and an enzyme substrate is added. The resultant colour intensity is proportional to the amount of collagen IV present in the sample. The assay range is 16 - 1000 ng/mL and the total assay incubation times are one hour.
Kit Inserts & MSDS
References
1. Obata, K. et al. (1989). One step sandwich immunoassay for human type IV collagen using monoclonal antibodies. Clin Chim Acta 181, 293-304.
2.Yokoya, Y. et al. (1992). Concentration of serum laminin and type IV collagen in liver disease assayed by a sandwich enzyme immunoassay using monoclonal antibodies. Clin Chim Acta 310, 109-118.
3. Matsumoto, E. et al. (1989). Increased amounts of serum type IV collagen in human liver fibrosis as determined by enzyme immunaoassay with monoclonal antibodies. Acta Pathol. Japan 39, 271-223.
4. Ueno,T. et al. (1992). Significance of serum type-IV collagen levels in various liver diseases. Scandinavian Journal of Gastroenterology 27, 513-520.
5. Tsursumi. M. et al. (1993). Changes in type IV collagen content in livers of patients with alcoholic liver disease. Hepatology 17, 820-827.
6. Yabu, K et al. (1994). Serum collagen IV for the assessment of fibrosis and resistance to interferon therapy in cronic hepatitis C. Scandinavian Journal of Gastroenterology 29, 474-479.
7. Matsumoto, E. et al. (1989). Increased serum type IV collagen peptide in carbon tetrachloride treated rats. Acta Pathol. Japan 39, 23-29.