Assay Principle

Argutus Medical Alpha GST EIA is a quantitative enzyme immunoassay. The test procedure is based on the sequential addition of sample, antibody-enzyme conjugate and substrate to microassay wells coated with anti-αGST IgG. The resultant colour intensity is proportional to the amount of αGST present in the sample.

Performance Characteristics

MEASURING RANGE
The calibration curve range is 2.5 - 80μg/L, which is equivalent to 6.25 - 200μg/L in stabilised urine samples diluted 1/2 in Sample Diluent or 12.5 - 400μg/L in serum/plasma samples diluted 1/5 in Sample Diluent.

SPECIFICITY
Argutus Medical Alpha GST EIA is highly specific for the detection of αGST. No cross-reactivity was observed with mu GST at 500µg/L, or pi GST at 500µg/L.

SENSITIVITY
The limit of detection (LoD) of Argutus Medical Alpha GST EIA was estimated from 60 blank sample measurements and 60 replicates of low-level sample measurements as per CLSI Document EP17-A. The limit of detection was found to be 1.9μg/L αGST, which corresponds to 4.75μg/L in a stabilised urine sample diluted 1/2 or 9.5μg/L in a serum/plasma sample diluted 1/5.
 

For more information on this product and it's usage see the links to product documentation on this page. Alternatively, feel free to contact us.