Why Use the Serum Collagen IV ELISA?
Chronic liver disease comprises a number of progressive disorders which culminate in liver cirrhosis and which are characterized by excessive deposition of collagen. Type IV collagen, a constituent of the basement membrane, is particularly noteworthy as:
- It's serum level correlates with hepatic levels of collagen IV
- Serum levels of collagen IV fall in response to effective therapy
- It is the earliest type of collagen to be synthesized during experimental liver injury.
Serum collagen IV levels are elevated in a variety of liver diseases and has to been found to be predictive of therapy response in Hepatitis C infection, and to be sensitive indicators of therapy response in abstaining alcoholics.
Serum Collagen IV ELISA - Assay Principle
The Argutus Medical Serum Collagen Type IV ELISA is designed for the assay of serum collagen type IV. It is a solid phase one-step sandwich ELISA assay. Collagen IV in the sample is bound simultaneously by a solid-phase monoclonal antibody and a monoclonal antibody-enzyme conjugate, each directed at different antigenic sites. This results in the collagen IV molecule being sandwiched between the solid phase and enzyme-labelled antibodies. After removing unbound enzyme-labelled antibody and sample, the plate is incubated with enzyme substrate. The resultant colour development is directly proportional to the amount of collagen IV in the sample.
Performance Characteristics
MEASURING RANGE
The calibration curve range covers the range 15.6-1000 μg/L. This range may be extended by increasing sample dilution.
SPECIFICITY
The Argutus Medical Serum Collagen EIA IV is highly specific for the detection of collagen IV. Cross reactivity is less than 2% with Collagen II and less than 0.5% with other forms of collagen.
SENSITIVITY
When reading from the standard curve the A450nm value of the 1000 μg/L standard should be >0.6 OD.
For more information on this Serum Collagen Type IV ELISA and it's usage see the links to product documentation on this page. Alternatively, feel free to contact us