Assay Principle for Urinary Collagen IV ELISA

The Argutus Medical Urinary Collagen Type IV ELISA Kit is designed for the assay of urinary collagen IV. It is a solid phase one-step sandwich ELISA. Collagen IV in the sample is bound simultaneously by a solid phase monoclonal antibody and a monoclonal antibody-enzyme conjugate directed at different antigenic sites. This results in the collagen IV molecule being sandwiched between the solid phase and enzyme labelled antibodies. After removing unbound enzyme labelled antibody and sample, the plate is then incubated with enzyme substrate, resulting in the development of a colour. The colour developed is directly proportional to the amount of collagen IV in the sample.

Performance Characteristics

MEASURING RANGE
The calibration curve range covers the range 0.8-50 μg/L. This range may be extended by increasing sample dilution.

SPECIFICITY
The Argutus Medical Urinary Collagen ELISA IV is highly specific for the detection of collagen IV. Cross reactivity is less than 2% with Collagen II and less than 0.5% with other forms of collagen.

SENSITIVITY
When reading from the standard curve the A450nm value of the 0.8 μg/L standard should be 0.01 – 0.06, and the 50 μg/L standard should be >0.8.
 

For more information on the Urinary Collagen Type IV ELISA Kit and it's usage see the links to product documentation on this page. Alternatively, feel free to contact us