Assay Principle

The Argutus Medical Urinary Collagen IV EIA is designed for the assay of urinary collagen IV. It is a solid phase one-step sandwich EIA. Collagen IV in the sample is bound simultaneously by a solid phase monoclonal antibody and a monoclonal antibody-enzyme conjugate directed at different antigenic sites. This results in the collagen IV molecule being sandwiched between the solid phase and enzyme labelled antibodies. After removing unbound enzyme labelled antibody and sample, the plate is then incubated with enzyme substrate, resulting in the development of a colour. The colour developed is directly proportional to the amount of collagen IV in the sample.

Performance Characteristics

MEASURING RANGE
The calibration curve range covers the range 0.8-50 μg/L. This range may be extended by increasing sample dilution.

SPECIFICITY
The Argutus Medical Urinary Collagen EIA IV is highly specific for the detection of collagen IV. Cross reactivity is less than 2% with Collagen II and less than 0.5% with other forms of collagen.

SENSITIVITY
When reading from the standard curve the A450nm value of the 0.8 μg/L standard should be 0.01 – 0.06, and the 50 μg/L standard should be >0.8.
 

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