Intended Use

This kit is capable of the quantitative determination of human L-FABP in urine.

Assay Principle

The procedure described here is an ELISA(Enzyme-Linked-Immuno-Sorbent Assay) of 2-step sandwich method.
L-FABP Standard or urine samples are firstly treated with Pretreatment Solution, and transferred into L-FABP Antibody Coated Microplate containing Assay Buffer and incubated. During this incubation, L-FABP in the reaction solution binds to the immobilized antibody. After washing, the 2nd Ab-POD Conjugate is added as the secondary antibody and incubated, thereby forming sandwich of the L-FABP antigen between the immobilized antibody and conjugate antibody.
After incubation, the plate is washed and added with Substrate for enzyme reaction, and then color develops according to the L-FABP antigen quantity. The optical density is measured using a microplate reader, and a calibration curve is prepared based on the obtained optical density, thereby determining the L-FABP concentration.

Performance Characteristics

SENSITIVITY
The sensitivity of the assay is 3ng/mL