Summary

• Elevated perfusate αGST can predict poor graft function.
• Perfusate αGST levels correlate with histological injury.
• Perfusate αGST can be used to compare graft storage and transport systems

1) Improved rat liver preservation by hypothermic continuous machine perfusion using Polysol, a new, enriched preservation solution
Bessems, M., Doorschodt, B.M., van Vliet, A.K. and van Gulik, T.M. (2005). Liver Transpl. 11(5) 539-46.

• Perfusate αGST levels were lower following perfusion with Polysol than with UW solution.

2) Value of α glutathione S-transferase for in-vitro evaluation of preservation injury in normal and steatotic livers.
Minor, T. et al. (2000). Transplant International 13 (Suppl. 1) 551-554.

• Isolated livers from 5 donor rats with fatty livers and 5 controls were perfused, then transplanted.
• αGST could identify those grafts at risk of failure.
• αGST was the most sensitive parameter for distinguishing between healthy and suboptimal grafts.
• αGST and GLDH was a particularly useful combination.

3) Value of alpha-glutathione sulfotransferase in the assessment of. hepatic injury after experimental long-term preservation.

Cascales, P., Fernández-Cornejo, V.J., Sánchez-del Campo, F., de Torre, M., Sánchez Eixeres, M.R., Soriano, N. and González F. (1999). Transplant Proc. 31(6) 2435-6.

• 23 rat livers studied.
• Perfusate αGST levels correlated more closely with histologically evaluated liver injury than ALT, AST and LDH.