Background | Applications | Intended Use | Principle of the Procedure | Performance Characteristics
Oxidative DNA Damage & Genotoxicity
The OxyDNA Test is a fast, convenient and sensitive fluorescence method-based genotoxicity assay kit which allows detection and quantification of oxidative DNA damage via the DNA adduct, 8-oxo-guanine (oxo8Gua, 8OG).
Oxidative DNA injury to macromolecules and cells, such as skin cells, is mediated via free oxygen radials also known as reactive oxygen species (ROS). Oxidative DNA damage is indicated in a wide range of pathological conditions. Free radicals may react causing reversible and irreversible oxidative DNA damage. This can lead to mutation, carcinogenesis, teratogenesis or cell death.
The probe in the OxyDNA Test is specific for 8-oxoguanine (8OG). 8-oxoguanine is formed during free radical damage to DNA and is a sensitive and specific indicator of DNA oxidation. It is a particularly important biomarker of oxidative DNA damage as it is formed in relatively large quantities.
8-oxoguanine formation can lead to mutations, such as the substitution of thymine for guanine and cytosine for adenine. Previously, 8-oxoguanine was difficult to detect, requiring HPLC analysis, however by utilizing a binding protein with high avidity and specificity for 8OG, as in the OxyDNA Test, detection of DNA damage can be achieved quickly and easily.
Oxidative DNA Damage is caused by a wide variety of agents and the OxyDNA test therefore has a wide range of applications in research.
Some Applications of OxyDNA Test
Skincare | Toxicology | Diabetes | Fertility | Cancer
Skincare Research
OxyDNA is a valuable tool for detection of DNA damage caused by UV radiation, useful for those studying DNA damage to skin cells such as keratinocytes. Skincare researchers evaluating the effectiveness of blocking agents in the development of sun creams for example will find the OxyDNA Test very useful.
See more publications referring to the use of OxyDNA in skincare research
Toxicology
OxyDNA can be used to detect toxicity in a range of cell culture systems including MDCK, neuronal and proximal tubule epithelial cells. OxyDNA can also be applied in environmental toxicology studies.
See papers featuring the use of OxyDNA in Toxicology studies and Environmental Toxicology
Diabetes Research
Using OxyDNA researchers have found diabetics have increased levels of oxidised DNA in peripheral lymphocytes, associated with shortened telomeres.
See more publications referring to the use of OxyDNA in diabetes research
Fertility Research & Monitoring
The OxyDNA Test has been used in a number of fertility studies. OxyDNA has helped researchers make new discoveries such as; cryopreservation of sperm increases oxidative DNA damage and increased staining with the OxyDNA kit is associated with decreased blastocyst viability.
See more publications referring to the use of OxyDNA in fertility research & monitoring
Cancer Research
OxyDNA is useful to those involved in cancer research and can be used to study oxidative DNA damage in a wide range of cancer models. Just some of these scientific publications can be found here.
Intended Use
The OxyDNA Test is an in-vitro fluorescent protein binding method for the detection of oxidative damage to DNA in fixed permeabilised cells using the Fluorescent Activated Cell Sorting (FACS) technique. Material of both human and animal origin can be tested.
Principle of the Procedure
The OxyDNA Test utilizes a direct fluorescent protein binding technique. After cells have been fixed and permeabilised, the FITC labeled protein conjugate is added and binds to the 8-oxoguanine moiety present in the 8-oxoguanosine of oxidized DNA. The presence of oxidized DNA is indicated by a green/yellow fluorescence that can be read using the Fluorescence Activated Cell Sorting procedure.
Performance Characteristics
The OxyDNA Test is specific for the 8-oxoguanine moiety of 8-oxoguanosine present in oxidized DNA and shows no measurable cross reactivity with unoxidised guanine / guanosine or other unoxidised nucleotides.
For more information on the OxyDNA Test and it's usage see the links to product documents on this page. Alternatively, feel free to contact us.