Assay Principle

Our Pi GST EIA is a quantitative enzyme immunoassay. The test procedure is based on the sequential addition of sample, antibody-enzyme conjugate and substrate to Microassay wells coated with anti-πGST IgG. The resultant colour intensity is proportional to the amount of πGST. The assay range is 1.25 - 40μg/L.

Performance Characteristics

MEASURING RANGE

The calibration curve range covers 1.25 - 40μg/L, corresponding to 3.13 - 100μg/L in stabilised urine samples diluted 1/2 in Sample Diluent. This range may be extended by increasing sample dilution.

SPECIFICITY
Our Pi GST EIA is highly specific for πGST. No cross-reactivity was observed with µGST at 500μg/L or αGST at 500μg/L.

SENSITIVITY
The limit of detection (LoD) of Argutus Medical Pi GST EIA was estimated from 60 blank sample measurements and 60 replicates of low-level sample measurements as per CLSI Document EP17-A. The limit of detection was found to be 1.3μg/L, which corresponds to a concentration of 3.25μg/L in a stabilised urine sample diluted 1/2.
 

For more information on this product and it's usage see the links to product documentation on this page. Alternatively, feel free to contact us.